Year: 2015 | Month: December | volume 2 | Issue 2

In vitro morphogenesis studies in gerbera jamesonii bolus ex hooker F.

Deepa Bhatt M.K. Tripathi Lal Singh* P.K.S. Gurjar A.K. Barholia Rajesh Jatav and Narendra Vasure
DOI:Coming Soon

Abstract:

An experiment was conducted during 2013 at the Horticultural Biotechnology Laboratory, KNK College of Horticulture, Mandsaur, RVSKVV, Gwalior (MP). The highest percentage of aseptic culture (83.54%) of explants were recorded with 0.5% bavistin in combination with 0.2% HgCl2 when diverse explants were exposed for 30 minutes. followed by application of 0.5% bavistin in combination with 0.1% HgCl2 (78.76%), while higher survival percentage (74.32%) of explants were evidenced with 0.5% bavistin in combination with 0.1% HgCl2 with exposure time 30 minutes. Combination of 0.5% bavistin with 0.1% HgCl2 exposed for the 30 minutes supported higher percentage of aseptic culture (78.76%) as well as survival of explants (74.13%) so that, this concentration and combination was used for surface sterilization of explants of gerbera during present investigation. The maximum callus was recorded on culture medium MS3D (35.09%), MS3N (33.83%) and MS2D (33.02%) with at par performance. Remaining culture media had low callus forming ability including MS5T (18.87%) that has significantly minimum counts. Maximum callus induction were recorded from cultured mature embryo on nutrients media MS3ip (35.06%) closely followed by a group of four nutrients media viz: MS2ip (29.95%), MSip (29.75%), MS.5ip (29.93%) and MS.2ip (29.12%). different culture media combination, inoculation media MS2N.5ip (55.22%), MS3N.5ip (53.17%) and MSN5ip (45.01%) were proved remarkably superior for shoots proliferating efficiency. Nutrients media MS5D.5Kn (3.05%) and MS4D.5KN (4.81%) was found low responding in this regard. Nutrient media MS2N (2.14cm), MSN (2.03cm) and MS2D (1.95) had produced shoot of higher length.



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